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Journal article

Transcriptionally active minichromosomes are attached transiently in nuclei through transcription units.

Abstract:

Viral minichromosomes provide simple models for chromatin domains. The sequences attaching them to larger nuclear structures were mapped; attachments were defined operationally by their ability to prevent chromatin fragments electroeluting from nuclei. Cells encapsulated in agarose microbeads were lysed and analysed subsequently in a 'physiological' buffer. Non-transcribed minichromosomes eluted from nuclei but transcriptionally active ones did not. Cutting attached minichromosomes with HaeII...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of cell science More from this journal
Volume:
105 ( Pt 4)
Pages:
1143-1150
Publication date:
1993-08-01
EISSN:
1477-9137
ISSN:
0021-9533
Language:
English
Keywords:
Pubs id:
pubs:18960
UUID:
uuid:f3db8f16-d438-46e6-8d55-b3f84168e869
Local pid:
pubs:18960
Source identifiers:
18960
Deposit date:
2012-12-19

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