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Fluorescence-Based Flow Sorting in Parallel with Transposon Insertion Site Sequencing Identifies Multidrug Efflux Systems in Acinetobacter baumannii

Abstract:

Multidrug efflux pumps provide clinically significant levels of drug resistance in a number of Gram-negative hospital-acquired pathogens. These pathogens frequently carry dozens of genes encoding putative multidrug efflux pumps. However, it can be difficult to determine how many of these pumps actually mediate antimicrobial efflux, and it can be even more challenging to identify the regulatory proteins that control expression of these pumps. In this study, we developed an innovative high-thro...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1128/mbio.01200-16

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Publisher:
American Society for Microbiology Publisher's website
Journal:
mBio Journal website
Volume:
7
Issue:
5
Pages:
e01200-16
Publication date:
2016-09-06
Acceptance date:
2016-08-12
DOI:
ISSN:
2150-7511
Pmid:
27601573
Source identifiers:
641723

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