Generating subclones from large-insert genomic clones.

Journal article

Abstract:: This unit provides a simple protocol for generating a partial-digest sublibrary of yeast DNA containing a YAC of interest. The starting material is high-molecular-weight chromosomal DNA embedded in agarose plugs. Many genome equivalents of these YAC subclones in bacteriophage or cosmid vectors can be plated and screened by hybridization with total human DNA to identify clones that originate from the human portion of the YAC. The human-positive clones can be picked into 96-well microtiter plat...
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Institution:

University of Oxford

Division:

MSD

Department:

NDM

Sub department:

Human Genetics Wt Centre

Role:

Author

Role:

Author

Journal:: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] More from this journal
Volume:: Chapter 5
Pages:: Unit-5.11
Publication date:: 2001-05-01
DOI:: 10.1002/0471142905.hg0511s03
EISSN:: 1934-8258
ISSN:: 1934-8266

Language:: English
Keywords:: DNA

DNA Fingerprinting

Genome, Human

Gene Library

Genetic Techniques

Bacteriophages

Chromosomes, Artificial, Yeast

Contig Mapping

Chromosomes, Human

Humans

Genomic Library

Genetic Vectors

Cosmids
Pubs id:: pubs:34208
UUID:: uuid:f1172e37-d27b-4d76-b538-24a011a632e8
Local pid:: pubs:34208
Source identifiers:: 34208
Deposit date:: 2012-12-19

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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