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Effects of storage conditions and digestion time on DNA amplification of 1 biting midge (Culicoides) blood meals

Abstract:

Background
Molecular analysis of blood meals is increasingly used to identify the hosts of biting insects such as midges and mosquitoes. Successful host identification depends on the availability of sufficient host DNA template for PCR amplification, making it important to understand how amplification success changes under different storage conditions and with different durations of blood meal digestion within the insect gut before being placed into the storage medium....

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/s13071-022-05607-x

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Role:
Author
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Institution:
University of Oxford
Role:
Author
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Institution:
University of Oxford
Role:
Author
Publisher:
BioMed Central
Journal:
Parasites and Vectors More from this journal
Volume:
16
Issue:
1
Article number:
13
Publication date:
2023-01-13
Acceptance date:
2022-12-02
DOI:
EISSN:
1756-3305
Language:
English
Keywords:
Pubs id:
1311333
Local pid:
pubs:1311333
Deposit date:
2022-12-05

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