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Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in Nicotiana benthamiana.

Abstract:

Agroinfiltrated Nicotiana benthamiana is a flexible and scalable platform for recombinant protein (RP) production, but its great potential is hampered by plant proteases that degrade RPs. Here, we tested 29 candidate protease inhibitors (PIs) in agroinfiltrated N. benthamiana leaves for enhancing accumulation of three unrelated RPs: glycoenzyme α-Galactosidase; glycohormone erythropoietin (EPO); and IgG antibody VRC01. Of the previously described PIs enhancing RP accumulation, we found only c...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1111/pbi.12916

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Plant Sciences
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Plant Sciences
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Plant Sciences
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Plant Sciences
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Plant Sciences
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Publisher:
Wiley Publisher's website
Journal:
Plant Biotechnology Journal Journal website
Volume:
16
Issue:
10
Pages:
1797-1810
Publication date:
2018-03-06
Acceptance date:
2018-02-28
DOI:
EISSN:
1467-7652
ISSN:
1467-7644
Pubs id:
pubs:828620
URN:
uri:f07531bd-4fa9-441c-be8d-ad850e74190d
UUID:
uuid:f07531bd-4fa9-441c-be8d-ad850e74190d
Local pid:
pubs:828620

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