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Journal article

Selective roles of vertebrate PCF11 in premature and full-length transcript termination

Abstract:

The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient termination. A key player in this process is the cleavage and polyadenylation (CPA) factor PCF11, which directly binds to the Pol II C-terminal domain and dismantles elongating Pol II from DNA in vitro. We demonstrate that PCF11-mediated termination is essential for vertebrate development. A range of genomic analyses, including mNET-seq, 3′ mRNA-seq, chromatin RNA-seq, and ChIP-seq, reveals that PCF11 enhances...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1016/j.molcel.2019.01.027

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0003-3789-5381
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
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Publisher:
Cell Press Publisher's website
Journal:
Molecular Cell Journal website
Volume:
74
Issue:
4
Pages:
158-172
Publication date:
2019-02-25
Acceptance date:
2019-01-17
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Pubs id:
pubs:980259
URN:
uri:eebcb1ba-a9d4-4e32-b1ce-85c1cfec684f
UUID:
uuid:eebcb1ba-a9d4-4e32-b1ce-85c1cfec684f
Local pid:
pubs:980259

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