Journal article
Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules
- Abstract:
- Unique molecular identifiers are random oligonucleotide sequences that remove PCR amplification biases. However, the impact that PCR associated sequencing errors have on the accuracy of generating absolute counts of RNA molecules is underappreciated. We show that PCR errors are a source of inaccuracy in both bulk and single-cell sequencing data, and synthesizing unique molecular identifiers using homotrimeric nucleotide blocks provides an error-correcting solution that allows absolute counting of sequenced molecules.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 3.4MB, Terms of use)
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- Publisher copy:
- 10.1038/s41592-024-02168-y
Authors
- Publisher:
- Springer Nature
- Journal:
- Nature Methods More from this journal
- Volume:
- 21
- Issue:
- 3
- Pages:
- 401–405
- Publication date:
- 2024-02-05
- Acceptance date:
- 2024-01-04
- DOI:
- EISSN:
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1548-7105
- ISSN:
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1548-7091
- Language:
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English
- Keywords:
- Pubs id:
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1616655
- Local pid:
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pubs:1616655
- Deposit date:
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2024-02-13
Terms of use
- Copyright holder:
- Sun et al
- Copyright date:
- 2024
- Rights statement:
- © The Author(s), under exclusive licence to Springer Nature America, Inc. 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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