A regulatory subunit of the cAMP-dependent protein kinase down-regulated in aplysia sensory neurons during long-term sensitization.

Journal article

Binding of cAMP by the five neuronal isoforms (N1-5) of the regulatory (R) subunit of the Aplysia cAMP-dependent protein kinase is diminished in sensory neurons stimulated to produce long-term presynaptic facilitation. To determine how the cAMP-binding activity of the R subunits is lost, we isolated cDNAs encoding N4, which is a homolog of mammalian RI. Immunoblots with antisera raised against the R protein overexpressed in E. coli show that the diminished binding activity, which occurs in lo...

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Authors: Bergold, P; Beushausen, SA; Sacktor, T; Cheley, S; Bayley, H

• Publication status: Published
• Journal: Neuron
• Volume: 8
• Issue: 2
• Pages: 387-397
• Publication date: 1992-02-01
• DOI: 10.1016/0896-6273(92)90304-v
• EISSN: 1097-4199
• ISSN: 0896-6273
• Language: English
• Keywords: Base Sequence; Sequence Homology, Nucleic Acid; Immunoblotting; Gene Expression Regulation, Enzymologic; Immune Sera; DNA; Protein Kinases; Macromolecular Substances; Isoenzymes; Animals; Time Factors; Cloning, Molecular; Cyclic AMP; Down-Regulation; Transcription, Genetic; Nucleic Acid Hybridization; Neurons, Afferent; Molecular Sequence Data; Amino Acid Sequence; Aplysia