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An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation

Abstract:

Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH). Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-type and mutant siblings can be done blinded and in parallel. Such experimental design reduces the technical variation between samples and minimises the risk of bias. This approach, however, requires...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1242/bio.031096

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
ORCID:
0000-0002-7708-3188
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Bonkhofer, F More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
ORCID:
0000-0002-1568-8165
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
ORCID:
0000-0002-4223-8506
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Funding agency for:
Dobrzycki, T
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Funding agency for:
Krecsmarik, M
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Funding agency for:
Monteiro, R
Publisher:
Company of Biologist Publisher's website
Journal:
Biology Open Journal website
Volume:
7
Issue:
4
Pages:
Article: bio031096
Publication date:
2018-04-09
Acceptance date:
2018-03-07
DOI:
ISSN:
2046-6390
Pubs id:
pubs:830319
URN:
uri:edc70bd1-41ca-4efe-8211-7663778efae2
UUID:
uuid:edc70bd1-41ca-4efe-8211-7663778efae2
Local pid:
pubs:830319
Language:
English
Keywords:

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