Influenza virus: a novel method to assess viral and neutralizing antibody titers in vitro.

Journal article

The present report describes novel in vitro assays to determine influenza virus titers and virus neutralizing antibody levels. For determination of viral titers, serial dilutions of influenza virus were incubated with MDCK-cells and cultured for 48 h under a methylcellulose overlay in 24 well plates. Cells were fixed, permeabilized and stained with a monoclonal antibody specific for hemagglutitin (HA) and a peroxidase labelled second stage antibody. The sensitivity of the assay was 100-1000 t...

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Authors: Bachmann, M; Ecabert, B; Kopf, M

• Publication status: Published
• Journal: Journal of immunological methods
• Volume: 225
• Issue: 1-2
• Pages: 105-111
• Publication date: 1999-05-01
• DOI: 10.1016/s0022-1759(99)00034-4
• EISSN: 1872-7905
• ISSN: 0022-1759
• Language: English
• Keywords: Mice, Inbred C57BL; Orthomyxoviridae; Neutralization Tests; Methods; Humans; Antibodies, Viral; Mice; Time Factors; Animals