Prospective technical validation and assessment of intra-tumour heterogeneity of a low density array hypoxia gene profile in head and neck squamous cell carcinoma.

Journal article

BACKGROUND AND PURPOSE: Tumour hypoxia is associated with a poor prognosis in head and neck squamous cell carcinoma (HNSCC), however there is no accepted method for assessing hypoxia clinically. We aimed to conduct a technical validation of a hypoxia gene expression signature using the TaqMan Low Density Array (TLDA) platform to investigate if this approach reliably identified hypoxic tumours. MATERIALS AND METHODS: Tumour samples (n=201) from 80 HNSCC patients were collected prospectively fr...

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Authors: Betts, G; Eustace, A; Patiar, S; Valentine, H; Irlam, J

• Publication status: Published
• Journal: European journal of cancer (Oxford, England : 1990)
• Volume: 49
• Issue: 1
• Pages: 156-165
• Publication date: 2013-01-01
• DOI: 10.1016/j.ejca.2012.07.028
• EISSN: 1879-0852
• ISSN: 0959-8049
• Language: English
• Keywords: Head and Neck Neoplasms; Neoplasm Staging; Transcriptome; Gene Expression Profiling; Carcinoma, Squamous Cell; Sensitivity and Specificity; Middle Aged; Aged; Female; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Cell Hypoxia; Real-Time Polymerase Chain Reaction; Humans; Adult; Immunohistochemistry; Male; Aged, 80 and over