Journal article icon

Journal article

Rapid and iterative genome editing in the malaria parasite Plasmodium knowlesi provides new tools for P. vivax research

Abstract:

Tackling relapsing Plasmodium vivax and zoonotic Plasmodium knowlesi infections is critical to reducing malaria incidence and mortality worldwide. Understanding the biology of these important and related parasites was previously constrained by the lack of robust molecular and genetic approaches. Here, we establish CRISPR-Cas9 genome editing in a culture-adapted P. knowlesi strain and define parameters for optimal homology-driven repair. We establish a scalable...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Publisher copy:
10.7554/eLife.45829

Authors


More by this author
Role:
Author
ORCID:
0000-0003-4726-6693
More from this funder
Name:
Lister Institute of Preventive Medicine
Grant:
Research Prize Fellow
More from this funder
Name:
Marshall Aid Commemoration Commission
Grant:
Marshall Scholarship
More from this funder
Name:
University of London
Grant:
Bloomsbury Colleges Research Studentship
More from this funder
Name:
Medical Research Council
Grant:
MRC Career Development Award MR/M021157/1
More from this funder
Name:
Biotechnology and Biological Sciences Research Council
Grant:
London Interdiscpinary Doctoral Programme Studentship
Publisher:
eLife Sciences Publications
Journal:
eLife More from this journal
Volume:
8
Article number:
e45829
Publication date:
2019-06-17
Acceptance date:
2019-05-28
DOI:
EISSN:
2050-084X
ISSN:
2050-084X
Pmid:
31205002
Language:
English
Keywords:
Pubs id:
pubs:1024435
UUID:
uuid:e8f754dc-0a2a-4c73-92d0-5c604ae10ca6
Local pid:
pubs:1024435
Source identifiers:
1024435
Deposit date:
2019-07-03

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP