Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast.

Journal article

Nucleotide synthesis is a universal response to DNA damage, but how this response facilitates DNA repair and cell survival is unclear. Here we establish a role for DNA damage-induced nucleotide synthesis in homologous recombination (HR) repair in fission yeast. Using a genetic screen, we found the Ddb1-Cul4(Cdt)² ubiquitin ligase complex and ribonucleotide reductase (RNR) to be required for HR repair of a DNA double-strand break (DSB). The Ddb1-Cul4(Cdt)² ubiquitin ligase complex is required ...

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Authors: Moss, J; Tinline-Purvis, H; Walker, C; Folkes, L; Stratford, MR

• Publication status: Published
• Journal: Genes and development
• Volume: 24
• Issue: 23
• Pages: 2705-2716
• Publication date: 2010-12-01
• DOI: 10.1101/gad.1970810
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: DNA Breaks, Double-Stranded; Gene Deletion; Schizosaccharomyces; Protein Kinases; Recombination, Genetic; DNA Repair; DNA-Binding Proteins; Nucleotides; Cell Cycle Proteins; Schizosaccharomyces pombe Proteins; Adaptor Proteins, Signal Transducing; Ribonucleotide Reductases; Checkpoint Kinase 2