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Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris.

Abstract:

We report the secreted expression by Pichia pastoris of two human lysozyme variants F57I and W64R, associated with systemic amyloid disease, and describe their characterization by biophysical methods. Both variants have a substantially decreased thermostability compared with wild-type human lysozyme, a finding that suggests an explanation for their increased propensity to form fibrillar aggregates and generate disease. The secreted yields of the F57I and W64R variants from P. pastoris are 200...

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Publication status:
Published

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Authors


Kumita, JR More by this author
Johnson, RJ More by this author
Alcocer, MJ More by this author
Dumoulin, M More by this author
Holmqvist, F More by this author
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Journal:
The FEBS journal
Volume:
273
Issue:
4
Pages:
711-720
Publication date:
2006-02-05
DOI:
EISSN:
1742-4658
ISSN:
1742-464X
URN:
uuid:e6abc05c-86f8-4f6b-86f9-d8df333727b2
Source identifiers:
59284
Local pid:
pubs:59284

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