Direct evidence for opposite effects of D-glucose and D-glyceraldehyde on cytoplasmic pH of mouse pancreatic beta-cells.

Journal article

The effects of D-glucose, D-glyceraldehyde, glibenclamide, D-600, NH4+ and high concentrations of K+ on cytoplasmic pH (pHi) were investigated in dispersed and cultured pancreatic beta-cells from ob/ob mice. The cytoplasmic pH was measured with the fluorescent H+-indicator quene 1. The average pHi value in resting beta-cells was 6.71. Addition of 20 mM of the physiological stimulus D-glucose increased pHi with 0.05 units. Both glibenclamide and high concentrations of K+ decreased pHi. The lat...

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Authors: Arkhammar, P; Berggren, P; Rorsman, P

• Publication status: Published
• Publisher: Kluwer Academic Publishers-Plenum Publishers
• Journal: Bioscience reports
• Volume: 6
• Issue: 4
• Pages: 355-361
• Publication date: 1986-04-01
• DOI: 10.1007/bf01116422
• EISSN: 1573-4935
• ISSN: 0144-8463
• Language: English
• Keywords: Islets of Langerhans; Glyceraldehyde; Glyburide; Glucose; Gallopamil; Female; Hydrogen-Ion Concentration; Mice, Obese; Animals; Male; Ammonia; Mice; Cytoplasm; Potassium