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Super-resolution measurement of distance between transcription sites using RNA FISH with intronic probes.

Abstract:

Nascent transcripts being copied from specific human genes can be detected using RNA FISH (fluorescence in situ hybridization) with intronic probes, and the distance between two different nascent transcripts is often measured when studying structure-function relationships. Such distance measurements are limited by the resolution of the light microscope. Here we describe methods for measuring these distances in cultured cells with a precision of a few tens of nanometers, using equipment found ...

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Publication status:
In press
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1016/j.ymeth.2015.11.009

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Publisher:
Elsevier Publisher's website
Journal:
Methods Journal website
Publication date:
2015-11-05
DOI:
EISSN:
1095-9130
ISSN:
1046-2023
URN:
uuid:e65a2f23-39e2-4481-a298-1ed0591cf86b
Source identifiers:
588903
Local pid:
pubs:588903

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