Poly(A) site selection in the HIV-1 provirus: inhibition of promoter-proximal polyadenylation by the downstream major splice donor site.

Journal article

In common with all retroviruses, the human immunodeficiency virus type 1 (HIV-1) contains duplicated long terminal repeat (LTR) sequences flanking the proviral genome. These LTRs contain identical poly(A) signals, which are both transcribed into RNA. Therefore, to allow efficient viral expression, a mechanism must exist to either restrict promoter-proximal poly(A) site use or enhance the activity of the promoter-distal poly(A) site. We have examined the use of both poly(A) sites using provira...

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Authors: Ashe, M; Griffin, P; James, W; Proudfoot, N

• Publication status: Published
• Journal: Genes and development
• Volume: 9
• Issue: 23
• Pages: 3008-3025
• Publication date: 1995-12-01
• DOI: 10.1101/gad.9.23.3008
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: Base Sequence; Gene Expression Regulation; Transcription, Genetic; Proviruses; Poly A; RNA Splicing; Mutation; Promoter Regions, Genetic; HIV-1; Binding, Competitive; RNA, Messenger; Humans; Molecular Sequence Data; HIV Long Terminal Repeat