Characterization of electron transfer dissociation in the Orbitrap Velos HCD cell.

Journal article

Electron transfer dissociation (ETD) is commonly employed in ion traps utilizing rf fields that facilitate efficient electron transfer reactions. Here, we explore performing ETD in the HCD collision cell on an Orbitrap Velos instrument by applying a static DC gradient axially to the rods. This gradient enables simultaneous three dimensional, charge sign independent, trapping of cations and anions, initiating electron transfer reactions in the center of the HCD cell where oppositely charged io...

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Authors: Frese, C; Nolting, D; Altelaar, A; Griep-Raming, J; Mohammed, S

• Publication status: Published
• Journal: Journal of the American Society for Mass Spectrometry
• Volume: 24
• Issue: 11
• Pages: 1663-1670
• Publication date: 2013-11-01
• DOI: 10.1007/s13361-013-0618-9
• EISSN: 1879-1123
• ISSN: 1044-0305
• Language: English
• Keywords: Electrons; Mass Spectrometry; Peptides; Tandem Mass Spectrometry; Cations; Peptide Fragments; Ions; Humans; Ubiquitin; Hela Cells