Strong cation exchange (SCX) based analytical methods for the targeted analysis of protein post-translational modifications.

Journal article

The multidimensional combination of strong cation exchange (SCX) chromatography and reversed phase chromatography has emerged as a powerful approach to separate peptides originating from complex samples such as digested cellular lysates or tissues before analysis by mass spectrometry, enabling the identification of over 10,000s of peptides and thousands of proteins in a single sample. Although, such multidimensional chromatography approaches are powerful, the in-depth analysis of protein post...

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Authors: Mohammed, S; Heck, A

• Publication status: Published
• Journal: Current opinion in biotechnology
• Volume: 22
• Issue: 1
• Pages: 9-16
• Publication date: 2011-02-01
• DOI: 10.1016/j.copbio.2010.09.005
• EISSN: 1879-0429
• ISSN: 0958-1669
• Language: English
• Keywords: Chromatography, Ion Exchange; Chemical Fractionation; Proteomics; Humans; Acetylation; Sequence Analysis, Protein; Protein Processing, Post-Translational; Mass Spectrometry; Chromatography, Reverse-Phase; Cations; Animals; Phosphorylation; Cation Exchange Resins; Proteins; Peptide Fragments