Separate quantification of doubly and singly 13C-labeled metabolites by HSQC-filtered J spectroscopy.
- NMR detection of multiply labeled compounds in biological samples is often used to follow metabolic pathways. Detection of protons bound to 13C atoms offers a more sensitive approach than direct 13C detection, but generally results in the loss of carbon-carbon coupling information. We have modified an HSQC sequence to refocus the carbon chemical shifts in order to obtain a proton-correlated 13C homonuclear J spectrum, which allows us to measure singly and doubly labeled compounds in the same spectrum.
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- Journal of magnetic resonance (San Diego, Calif. : 1997)
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