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Separate quantification of doubly and singly 13C-labeled metabolites by HSQC-filtered J spectroscopy.

Abstract:
NMR detection of multiply labeled compounds in biological samples is often used to follow metabolic pathways. Detection of protons bound to 13C atoms offers a more sensitive approach than direct 13C detection, but generally results in the loss of carbon-carbon coupling information. We have modified an HSQC sequence to refocus the carbon chemical shifts in order to obtain a proton-correlated 13C homonuclear J spectrum, which allows us to measure singly and doubly labeled compounds in the same spectrum.
Publication status:
Published

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Publisher copy:
10.1006/jmre.1998.1698

Authors


Davison, AP More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Atomic and Laser Physics
Journal:
Journal of magnetic resonance (San Diego, Calif. : 1997)
Volume:
137
Issue:
2
Pages:
448-450
Publication date:
1999-04-05
DOI:
EISSN:
1096-0856
ISSN:
1090-7807
URN:
uuid:e574812b-bb41-476c-b702-817febea8f2a
Source identifiers:
11672
Local pid:
pubs:11672

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