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Asymmetric and functional reconstitution of band 3 into pre-formed phosphatidylcholine vesicles.

Abstract:

Human erythrocyte band 3 protein was purified in 0.1% Triton X-100 and reconstituted into pre-formed phosphatidylcholine vesicles by a Triton X-100-mediated procedure [1]. Band 3 (and its transmembrane domain) could be asymmetrically reconstituted into phosphatidylcholine vesicles with retention of sulfate transport activity which showed behaviour characteristic of red cell anion transport in response to pH, H2DIDS and temperature. Successful reconstitution was also possible using high mol ra...

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Publication status:
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Authors


Boulter, JM More by this author
Taylor, AM More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Journal:
Biochimica et biophysica acta
Volume:
1280
Issue:
2
Pages:
265-271
Publication date:
1996-04-05
DOI:
ISSN:
0006-3002
URN:
uuid:e52cdd96-8455-46b0-bbec-51279ab24e29
Source identifiers:
410500
Local pid:
pubs:410500

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