A secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice.

Journal article

In stable transfection experiments in the GH-producing GC cell line, a construct containing the entire signal peptide and the first 22 residues of human GH linked in frame with enhanced green fluorescent protein (eGFP), produced brightly fluorescent cells with a granular distribution of eGFP. This eGFP reporter was then inserted into a 40-kb cosmid transgene containing the locus control region for the hGH gene and used to generate transgenic mice. Anterior pituitaries from these GH-eGFP trans...

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Authors: Magoulas, C; McGuinness, L; Balthasar, N; Carmignac, D; Sesay, A

• Publication status: Published
• Journal: Endocrinology
• Volume: 141
• Issue: 12
• Pages: 4681-4689
• Publication date: 2000-12-01
• DOI: 10.1210/en.141.12.4681
• EISSN: 1945-7170
• ISSN: 0013-7227
• Language: English
• Keywords: Humans; Green Fluorescent Proteins; Pituitary Gland, Anterior; Animals; Growth Hormone-Releasing Hormone; Calcium; Cosmids; Cytoplasmic Granules; Cytosol; Human Growth Hormone; Mice; Luminescent Proteins; Microscopy, Confocal; Flow Cytometry; Microscopy, Immunoelectron; Immunohistochemistry; Microscopy, Fluorescence; Gene Expression; Mice, Transgenic