Regional and temporal specialization in the nucleus: a transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle.

Journal article

PTF (PSE-binding transcription factor) activates transcription of snRNA and related genes. We investigated its distribution in HeLa nuclei by immunofluorescence, and found it spread throughout the nucleoplasm in small foci. In some cells, PTF is also concentrated in one, or very few, discrete regions (diameter approximately 1.3 micron) that appear during G1 phase and disappear in S phase. Oct1, a transcription factor that interacts with PTF, is also enriched in these domains; RNA polymerase I...

Expand abstract

Authors: Pombo, A; Cuello, P; Schul, W; Yoon, J; Roeder, R

• Publication status: Published
• Journal: EMBO journal
• Volume: 17
• Issue: 6
• Pages: 1768-1778
• Publication date: 1998-03-01
• DOI: 10.1093/emboj/17.6.1768
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Amanitins; Cell Cycle; Chromosomes, Human; Hela Cells; Transcription, Genetic; Dichlororibofuranosylbenzimidazole; Host Cell Factor C1; DNA-Binding Proteins; Homeodomain Proteins; Antigens, Nuclear; Nuclear Proteins; Transcription Factors; Humans; Octamer Transcription Factor-1; RNA Polymerase II; Cell Nucleus; Nucleic Acid Synthesis Inhibitors