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Sensing nitrite through a pseudoazurin-nitrite reductase electron transfer relay.

Abstract:

Nitrite is converted to nitric oxide by haem or copper-containing enzymes in denitrifying bacteria during the process of denitrification. In designing an efficient biosensor, this enzymic turnover must be quantitatively assessed. The enzyme nitrite reductase from Alcaligenes faecalis contains a redox-active blue copper centre and a nonblue enzyme-active copper centre. It can be covalently tethered to modified gold-electrode surfaces in configurations in which direct electron transfer is possi...

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Publication status:
Published

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Publisher copy:
10.1002/cphc.200400384

Authors


Canters, GW More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Verbeet, MP More by this author
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Journal:
Chemphyschem : a European journal of chemical physics and physical chemistry
Volume:
6
Issue:
6
Pages:
1114-1120
Publication date:
2005-06-05
DOI:
EISSN:
1439-7641
ISSN:
1439-4235
URN:
uuid:e40d93ba-f631-40e3-9b45-012fa4d746e8
Source identifiers:
33102
Local pid:
pubs:33102

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