Quantification of the islet product: presentation of a standardized current good manufacturing practices compliant system with minimal variability.

Journal article

BACKGROUND: Accurate islet quantification has proven difficult to standardize in a good manufacturing practices (GMP) approved manner. METHODS: The influence of assessment variables from both manual and computer-assisted digital image analysis (DIA) methods were compared using calibrated, standardized microspheres or islets alone. Additionally, a mixture of microspheres and exocrine tissue was used to evaluate the variability of both the current, internationally recognized, manual method and...

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Authors: Friberg, A; Brandhorst, H; Buchwald, P; Goto, M; Ricordi, C

• Journal: Transplantation
• Volume: 91
• Issue: 6
• Pages: 677-683
• Publication date: 2011-03-01
• DOI: 10.1097/tp.0b013e31820ae48e
• EISSN: 1534-6080
• ISSN: 0041-1337
• Language: English
• Keywords: Humans; Islets of Langerhans; Islets of Langerhans Transplantation; Reproducibility of Results; Calibration; Image Processing, Computer-Assisted; Cell Size; Microspheres