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Journal article

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.

Abstract:
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.
Publication status:
Published

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Publisher copy:
10.1038/nbt1399

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, RDM Clinical Laboratory Sciences, Biomedical Research Centre
Pringle, IA More by this author
Abdullah, S More by this author
Lawton, AE More by this author
Davies, LA More by this author
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Journal:
Nature biotechnology
Volume:
26
Issue:
5
Pages:
549-551
Publication date:
2008-05-05
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
URN:
uuid:e2c6fa2d-6ce3-4bd4-a1a2-177fd0f70cbf
Source identifiers:
246534
Local pid:
pubs:246534

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