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Glutamine-330 is not essential for activity in isopenicillin N synthase from Aspergillus nidulans.

Abstract:

The non-heme ferrous dependent oxidase isopenicillin N synthase (IPNS) catalyses the biosynthesis of isopenicillin N from a tripeptide substrate. The crystal structure of Aspergillus nidulans IPNS complexed to manganese reveals a six co-ordinate metal ligated by two water molecules and four protein ligands: His-214, His-270, Asp-216 and Gln-330 (the penultimate C-terminal residue). Modification of Gln-330 to Ala or Leu, or deletion of 2 or 6 residues from the C-terminus resulted in lowering o...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Role:
Author
Journal:
FEBS letters
Volume:
405
Issue:
2
Pages:
191-194
Publication date:
1997-03-05
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
URN:
uuid:e28c3fa1-febb-4b09-8ecf-5b2c9b9dfb02
Source identifiers:
36037
Local pid:
pubs:36037

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