Adrenaline and extracellular ATP switch between two modes of acid extrusion in the guinea-pig ventricular myocyte.

Journal article

1. Intracellular pH (pHi) was recorded in isolated guinea-pig ventricular myocytes using the pH-sensitive fluoroprobe, carboxy-SNARF-1 (carboxy-seminaphthorhodafluor). 2. Addition and removal of 10 mM NH4Cl was used to induce an intracellular acid load in a myocyte perfused with HCO3(-)-buffered solution containing amiloride. Under these conditions, subsequent pHi recovery is known to rely upon Na(+)-HCO3- co-transport into the cell. The application of 0.5-5 microM adrenaline resulted in an i...

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Authors: Lagadic-Gossmann, D; Vaughan-Jones, R; Buckler, K

• Publication status: Published
• Journal: Journal of physiology
• Volume: 458
• Pages: 385-407
• Publication date: 1992-12-01
• EISSN: 1469-7793
• ISSN: 0022-3751
• Language: English
• Keywords: Animals; Hydrogen-Ion Concentration; Sodium-Potassium-Exchanging ATPase; Membrane Potentials; Proton Pumps; Fluorescent Dyes; Myocardium; Benzopyrans; Guinea Pigs; Ion Transport; Adenosine Triphosphate; Rhodamines; Naphthols; Epinephrine; Bicarbonates