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Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detection.

Abstract:

A study of the practicality a simple technique for obtaining time-domain information that uses continuous wave detection of fluorescence is presented. We show that this technique has potential for use in assays for which a change in the lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer, for example, and could be particularly important when one is carrying out such measurements in the scaled-down environment of a lab on a chip (biochip). A rat...

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Publication status:
Published

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Publisher copy:
10.1364/ao.45.002115

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Publisher:
Optical Society of American (OSA)
Journal:
Applied optics
Volume:
45
Issue:
9
Pages:
2115-2123
Publication date:
2006-03-05
DOI:
EISSN:
1539-4522
ISSN:
0003-6935
URN:
uuid:e19a0a1a-51b4-48df-9638-404bd77a75e2
Source identifiers:
130864
Local pid:
pubs:130864

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