Detection of fetal cells in maternal blood.

Journal article

We report the detection of fetal cells in the maternal circulation by enzymatic amplification of a single copy gene sequence that was fetal-specific. Fetal HLA-A2-positive cells were sorted from maternal HLA-A2-negative cells by flow cytometry and confirmed by demonstration of a fetal-specific HLA-DR4 sequence. However, this sequence could not be detected in unenriched maternal DNA prepared at 28 and 32 weeks' gestation. The sensitivity of detection was 1 HLA-DR4-positive cell in 10(5) HLA-DR...

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Authors: Yeoh, S; Sargent, I; Redman, C; Wordsworth, B; Thein, S

• Publication status: Published
• Journal: Prenatal diagnosis
• Volume: 11
• Issue: 2
• Pages: 117-123
• Publication date: 1991-02-01
• DOI: 10.1002/pd.1970110207
• EISSN: 1097-0223
• ISSN: 0197-3851
• Language: English
• Keywords: Female; Flow Cytometry; HLA-DR4 Antigen; Humans; HLA-A2 Antigen; Gene Amplification; Pregnancy; HLA-DR Antigens; Oligonucleotide Probes; Polymerase Chain Reaction; Fetus; Nucleic Acid Hybridization