Type II regulatory subunits of cAMP-dependent protein kinase and their binding proteins in the nervous system of Aplysia californica.

Journal article

Two type II regulatory (R) subunits of cAMP-dependent protein kinase (PKA) of 50 and 47 kDa have been identified in Aplysia neurons by several criteria which include phosphorylation by the catalytic subunit of PKA and nanomolar affinity for a peptide fragment of the human thyroid protein Ht 31, properties that in mammals distinguish type II from type I R subunits. The neuronal type II R subunits are differentially localized within cells. For example, the 50-kDa polypeptide is enriched in taxo...

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Authors: Cheley, S; Panchal, R; Carr, D; Scott, J; Bayley, H

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 269
• Issue: 4
• Pages: 2911-2920
• Publication date: 1994-01-01
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Humans; Cyclic AMP-Dependent Protein Kinase Type II; Neurons; Electrophoresis, Polyacrylamide Gel; Cyclic AMP-Dependent Protein Kinases; Aplysia; Subcellular Fractions; Muscles; Molecular Weight; Cattle; Nervous System; Cell Line; Transfection; Phosphoproteins; Animals; Moths; Nerve Tissue Proteins; Ganglia; Recombinant Proteins; Microtubules; Paclitaxel; Restriction Mapping