Journal article icon

Journal article

Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.

Abstract:

We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluoresc...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.1038/srep42398

Authors


Expand authors...
More from this funder
Funding agency for:
Daniels, MJ
More from this funder
Funding agency for:
Daniels, MJ
Japan Science and Technology Agency More from this funder
Publisher:
Nature Publishing Group Publisher's website
Journal:
Scientific Reports Journal website
Volume:
7
Pages:
42398
Publication date:
2017-02-05
Acceptance date:
2017-01-09
DOI:
ISSN:
2045-2322
Pubs id:
pubs:681732
URN:
uri:e07f1142-c13b-48c4-8fb5-bcf7292de87f
UUID:
uuid:e07f1142-c13b-48c4-8fb5-bcf7292de87f
Local pid:
pubs:681732

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP