- Abstract:
-
We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluoresc...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 1.1MB)
-
- Publisher copy:
- 10.1038/srep42398
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- Grant:
- 3306
- Publisher:
- Nature Publishing Group Publisher's website
- Journal:
- Scientific Reports Journal website
- Volume:
- 7
- Pages:
- 42398
- Publication date:
- 2017-02-05
- Acceptance date:
- 2017-01-09
- DOI:
- ISSN:
-
2045-2322
- Pubs id:
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pubs:681732
- URN:
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uri:e07f1142-c13b-48c4-8fb5-bcf7292de87f
- UUID:
-
uuid:e07f1142-c13b-48c4-8fb5-bcf7292de87f
- Local pid:
- pubs:681732
- Language:
- English
- Keywords:
- Copyright holder:
- Daniels et al
- Copyright date:
- 2017
- Notes:
- © The Author(s) 2017 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
Journal article
Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.
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+ Japanese Ministry of Education, Culture, Sports, Science and Technology
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Japan Science and Technology Agency
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