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Journal article

A simple, safe and sensitive method for SARS-CoV-2 inactivation and RNA extraction for RT-qPCR

Abstract:

The SARS‐CoV‐2 pandemic has created an urgent need for diagnostic tests to detect viral RNA. Commercial RNA extraction kits are often expensive, in limited supply, and do not always fully inactivate the virus. Together, this calls for the development of safer methods for SARS‐CoV‐2 extraction that utilize readily available reagents and equipment present in most standard laboratories. We optimized and simplified a RNA extraction method combining a high molar acidic guanidinium isothiocyanate (...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1111/apm.13123

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Publisher:
Wiley Publisher's website
Journal:
APMIS Journal website
Volume:
129
Issue:
7
Pages:
393-400
Publication date:
2021-03-17
Acceptance date:
2021-02-02
DOI:
EISSN:
1600-0463
ISSN:
0903-4641
Pubs id:
1169563
Local pid:
pubs:1169563
Language:
English
Keywords:

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