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A versatile strategy for production of membrane proteins with diverse topologies: application to investigation of bacterial homologues of human divalent metal ion and nucleoside transporters

Abstract:

Membrane proteins play key roles in many biological processes, from acquisition of nutrients to neurotransmission, and are targets for more than 50% of current therapeutic drugs. However, their investigation is hampered by difficulties in their production and purification on a scale suitable for structural studies. In particular, the nature and location of affinity tags introduced for the purification of recombinant membrane proteins can greatly influence their expression levels by affecting ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1371/journal.pone.0143010

Authors


Huysmans, G More by this author
Bullough, P More by this author
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Publisher:
Public Library of Science Publisher's website
Journal:
PLOS One Journal website
Volume:
10
Issue:
11
Pages:
Article: e0143010
Publication date:
2015
DOI:
EISSN:
1932-6203
URN:
uuid:e0195641-8b05-4095-b70f-40ae4a36fce3
Source identifiers:
596866
Local pid:
pubs:596866

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