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Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

Abstract:
When imaging through tissue, the optical inhomogeneities of the sample generate aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This is particularly problematic for 3D-enhanced STED. We present here an adaptive optics implementation that incorporates two adaptive optic elements to enable correction in all beam paths, allowing performance improvement in thick tissue samples. We use this to demonstrate 3D STED imaging of complex structures in Drosophila melanogaster brains.
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1364/OE.24.008862

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
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Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
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Funding agency for:
Patton, BR
Publisher:
Optical Society of America Publisher's website
Journal:
Optics Express Journal website
Volume:
24
Pages:
8862-8876
Publication date:
2016-04-17
Acceptance date:
2016-03-26
DOI:
ISSN:
1094-4087
URN:
uuid:df8fa48c-bd2f-4e06-9c12-1c2dcb950184
Source identifiers:
613776
Local pid:
pubs:613776
Paper number:
8

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