Journal article
Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics
- Abstract:
- When imaging through tissue, the optical inhomogeneities of the sample generate aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This is particularly problematic for 3D-enhanced STED. We present here an adaptive optics implementation that incorporates two adaptive optic elements to enable correction in all beam paths, allowing performance improvement in thick tissue samples. We use this to demonstrate 3D STED imaging of complex structures in Drosophila melanogaster brains.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 4.3MB, Terms of use)
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- Publisher copy:
- 10.1364/OE.24.008862
Authors
Funding
+ Wellcome Trust Society
More from this funder
Funding agency for:
Owald, D
Grant:
Sir Henry Wellcome Fellowship
Bibliographic Details
- Publisher:
- Optical Society of America
- Journal:
- Optics Express More from this journal
- Volume:
- 24
- Pages:
- 8862-8876
- Publication date:
- 2016-04-01
- Acceptance date:
- 2016-03-26
- DOI:
- ISSN:
-
1094-4087
Item Description
- Keywords:
- Pubs id:
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pubs:613776
- UUID:
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uuid:df8fa48c-bd2f-4e06-9c12-1c2dcb950184
- Local pid:
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pubs:613776
- Source identifiers:
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613776
- Deposit date:
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2016-04-05
Terms of use
- Copyright holder:
- Optical Society of America
- Copyright date:
- 2016
- Notes:
- © 2016 OSA. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
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