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Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: a real time scanning tunnelling microscopy study.

Abstract:

A scanning tunnelling microscopy study of adsorption of wild-type Pseudomonas putida putidaredoxin at a gold (111)-buffer interface has been made in real time. Reversible adsorption has been observed reflecting weak interaction of the wild-type protein with a gold (111) electrode. A genetically engineered mutant, C73S-D58C, which contains a surface thiol, has been used for 'immobilization' and 'orientated adsorption' on the gold surface. The implication of such orientated immobilization in de...

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Publication status:
Published

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Publisher copy:
10.1111/j.1365-2818.2004.01246.x

Authors

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
Journal:
Journal of microscopy More from this journal
Volume:
213
Issue:
Pt 1
Pages:
6-10
Publication date:
2004-01-01
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
Language:
English
Keywords:
Pubs id:
pubs:32577
UUID:
uuid:df8b9876-3884-44bf-9e17-00737fdb2d8f
Local pid:
pubs:32577
Source identifiers:
32577
Deposit date:
2012-12-19

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