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Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: a real time scanning tunnelling microscopy study.

Abstract:

A scanning tunnelling microscopy study of adsorption of wild-type Pseudomonas putida putidaredoxin at a gold (111)-buffer interface has been made in real time. Reversible adsorption has been observed reflecting weak interaction of the wild-type protein with a gold (111) electrode. A genetically engineered mutant, C73S-D58C, which contains a surface thiol, has been used for 'immobilization' and 'orientated adsorption' on the gold surface. The implication of such orientated immobilization in de...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Role:
Author
Journal:
Journal of microscopy
Volume:
213
Issue:
Pt 1
Pages:
6-10
Publication date:
2004-01-05
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
URN:
uuid:df8b9876-3884-44bf-9e17-00737fdb2d8f
Source identifiers:
32577
Local pid:
pubs:32577

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