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A protocol for extraction of total RNA from finger stick whole blood samples preserved with TempusTM solution

Abstract:

Monitoring of blood transcriptional changes during disease or treatment could improve the understanding of cellular mechanisms associated with that particular condition. This can be achieved through serial sampling of small blood volumes. However, molecular analysis of gene expression from low volume samples remains a challenging task. To address this issue, we have developed a set of standard operating procedures (SOP), starting from collection of small volume blood to measurement of gene ex...

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Publication status:
Published
Peer review status:
Under review
Version:
Publisher's version

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Publisher copy:
10.12688/f1000research.16622.1

Authors


Syed Ahamed Kabeer, B More by this author
Brummaier, T More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Tropical Medicine
ORCID:
0000-0003-1621-3257
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Qatar Foundation More from this funder
Publisher:
F1000Research Publisher's website
Journal:
F1000Research Journal website
Publication date:
2018-11-02
Acceptance date:
2018-11-02
DOI:
Pubs id:
pubs:942211
URN:
uri:df0b7f0b-1fcf-4cf5-8173-baeebdd89b49
UUID:
uuid:df0b7f0b-1fcf-4cf5-8173-baeebdd89b49
Local pid:
pubs:942211

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