In-situ analysis of calcitonin and CGRP expression in medullary thyroid carcinoma.

Journal article

A combination of immunocytochemistry (ICC) and in-situ hybridization (ISH) applied to formalin-fixed tissue sections was used to analyse the differential expression of calcitonin and CGRP genes, at both peptide and mRNA levels, in normal and neoplastic human thyroid C cells. Calcitonin peptide was readily detectable in normal C cells but its abundance in the neoplastic C cells of medullary thyroid carcinoma (MTC) was reduced in correlation with the degree of tumour differentiation. Conversely...

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Authors: Boultwood, J; Wynford-Thomas, D; Richards, G; Craig, R; Williams, E

• Journal: Clinical endocrinology
• Volume: 33
• Issue: 3
• Pages: 381-390
• Publication date: 1990-09-01
• DOI: 10.1111/j.1365-2265.1990.tb00503.x
• EISSN: 1365-2265
• ISSN: 0300-0664
• Language: English
• Keywords: RNA Probes; Adult; Thyroid Gland; Transcription, Genetic; Immunohistochemistry; Calcitonin Gene-Related Peptide; Molecular Probe Techniques; Humans; Aged; Thyroid Neoplasms; Carcinoma; Middle Aged; Calcitonin; RNA, Messenger