Journal article icon

Journal article

Use of a phage display library to identify oligopeptides binding to the lumenal surface of polarized endothelium by ex vivo perfusion of human umbilical veins.

Abstract:

Human endothelial-specific targeting peptides were identified by biopanning within freshly-obtained human umbilical cords. Umbilical veins were cleaned in situ and M13 phage display libraries were passed through the cords. Tightly bound phage were recovered following isolation of endothelial cells by collagenase digestion and homogenisation, allowing production of enriched phage libraries for subsequent rounds of panning. After five rounds of biopanning, five promising sequences were selected...

Expand abstract

Actions


Access Document


Publisher copy:
10.1080/1061186031000086063

Authors


Parker, AL More by this author
Fisher, KD More by this author
Murray, PG More by this author
Expand authors...
Journal:
Journal of drug targeting
Volume:
11
Issue:
1
Pages:
53-59
Publication date:
2003-01-05
DOI:
EISSN:
1029-2330
ISSN:
1061-186X
URN:
uuid:de5f9457-99e8-413a-a6ea-83651bd159b8
Source identifiers:
178193
Local pid:
pubs:178193

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP