Traveling-wave ion mobility mass spectrometry of protein complexes: accurate calibrated collision cross-sections of human insulin oligomers.

Journal article

RATIONALE: The collision cross-section (Ω) of a protein or protein complex ion can be measured using traveling-wave (T-wave) ion mobility (IM) mass spectrometry (MS) via calibration with compounds of known Ω. The T-wave Ω-values depend strongly on instrument parameters and calibrant selection. Optimization of instrument parameters and calibration standards are crucial for obtaining accurate T-wave Ω-values. METHODS: Human insulin and the fast-acting insulin aspart under native-like condition...

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Authors: Salbo, R; Bush, M; Naver, H; Campuzano, I; Robinson, C

• Publication status: Published
• Journal: Rapid communications in mass spectrometry : RCM
• Volume: 26
• Issue: 10
• Pages: 1181-1193
• Publication date: 2012-05-01
• DOI: 10.1002/rcm.6211
• EISSN: 1097-0231
• ISSN: 0951-4198
• Language: English
• Keywords: Mass Spectrometry; Insulin Aspart; Insulin, Regular, Human; Humans; Ions; Linear Models; Protein Subunits; Calibration