Novel protein phosphorylation site identification in spinach stroma membranes by titanium dioxide microcolumns and tandem mass spectrometry.

Journal article

In this work, spinach stroma membrane, instead of thylakoid, has been investigated for the presence of phosphorylated proteins. We identified seven previously unknown phosphorylation sites by taking advantage of TiO(2) phosphopeptides enrichment coupled to mass spectrometric analysis. Upon illumination at 100 micromol m(-2) s(-1), two novel phosphopeptides belonging to the N-terminal region of Lhcb1 light-harvesting protein were detected: NVSSGS(p)PWYGPDR and T(p)VQSSSPWYGPDR. Moreover, three...

Expand abstract

Authors: Rinalducci, S; Larsen, MR; Mohammed, S; Zolla, L

• Publication status: Published
• Journal: Journal of proteome research
• Volume: 5
• Issue: 4
• Pages: 973-982
• Publication date: 2006-04-01
• DOI: 10.1021/pr050476n
• EISSN: 1535-3907
• ISSN: 1535-3893
• Language: English
• Keywords: Titanium; Stromal Cells; Mass Spectrometry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Cell Fractionation; Cell Membrane; Chromatography, Liquid; Subcellular Fractions; Nanotechnology; Phosphorylation; Plant Leaves; Spinacia oleracea; Plant Proteins; Chlorophyll