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Demultiplexing of single-cell RNA-sequencing data using interindividual variation in gene expression

Abstract:

Motivation: Pooled designs for single-cell RNA sequencing, where many cells from distinct samples are processed jointly, offer increased throughput and reduced batch variation. This study describes expression-aware demultiplexing (EAD), a computational method that employs differential co-expression patterns between individuals to demultiplex pooled samples without any extra experimental steps. Results: We use synthetic sample pools and show that the top interindividual differentially co-expre...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/bioadv/vbae085

Authors


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Institution:
University of Oxford
Role:
Author
ORCID:
0000-0003-1886-1151
More by this author
Institution:
University of Oxford
Role:
Author
ORCID:
0000-0001-8928-5054
More by this author
Institution:
University of Oxford
Role:
Author
More by this author
Institution:
University of Oxford
Role:
Author
More by this author
Institution:
University of Oxford
Role:
Author
Publisher:
Oxford University Press
Journal:
Bioinformatics Advances More from this journal
Volume:
4
Issue:
1
Article number:
vbae085
Publication date:
2024-06-08
Acceptance date:
2024-06-07
DOI:
EISSN:
2635-0041
Language:
English
Source identifiers:
2060106
Deposit date:
2024-06-22

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