Characterization of a panel of novel anti-p21Waf1/Cip1 monoclonal antibodies and immunochemical analysis of p21Waf1/Cip1 expression in normal human tissues.

Journal article

As a universal inhibitor of cyclin-dependent kinases and one of the target genes of the tumor suppresser p53, p21Waf1/Cip1 can act as a tumor suppresser through its ability to control cell cycle progression. To study the function of p21Waf1/Cip1 protein and to investigate its tissue distribution, a panel of anti-p21Waf1/Cip1 monoclonal antibodies was generated. These anti-p21Waf1/Cip1 monoclonal antibodies were initially raised against a GST-p21Waf1/Cip1 fusion protein produced in bacteria. D...

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Authors: Fredersdorf, S; Milne, A; Hall, P; Lu, X

• Publication status: Published
• Journal: American journal of pathology
• Volume: 148
• Issue: 3
• Pages: 825-835
• Publication date: 1996-03-01
• EISSN: 1525-2191
• ISSN: 0002-9440
• Language: English
• Keywords: Humans; Tumor Cells, Cultured; Antibodies, Monoclonal; Immunoblotting; Recombinant Proteins; Gene Expression Regulation; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; Osteosarcoma; Reference Values; Amino Acid Sequence; Precipitin Tests; Molecular Sequence Data; Immunochemistry