Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate.

Journal article

BACKGROUND AND PURPOSE: To validate a fluorescence approach for monitoring norepinephrine transporter (NET) transport rate in mature sympathetic terminals, and to determine how prejunctional muscarinic receptors affect NET rate. EXPERIMENTAL APPROACH: Confocal imaging of a fluorescent NET substrate [neurotransmitter transporter uptake assay (NTUA)] as it accumulates in the mature sympathetic nerve terminals of the mouse isolated vas deferens. Fluorescence recovery after photobleaching (FRAP),...

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Authors: Parker, L; Shanks, J; Kennard, J; Brain, K

• Publication status: Published
• Journal: British journal of pharmacology
• Volume: 159
• Issue: 4
• Pages: 797-807
• Publication date: 2010-02-01
• DOI: 10.1111/j.1476-5381.2009.00574.x
• EISSN: 1476-5381
• ISSN: 0007-1188
• Language: English
• Keywords: Mice, Transgenic; Animals; Microscopy, Confocal; Organic Chemicals; Muscle Contraction; Molecular Probe Techniques; Cholinergic Fibers; Choline O-Acetyltransferase; Mice; Adrenergic Uptake Inhibitors; Desipramine; Receptors, Presynaptic; Green Fluorescent Proteins; Fluorescence Recovery After Photobleaching; Vas Deferens; Fluorescent Dyes; Molecular Imaging; Reproducibility of Results; Receptors, Muscarinic; Male; Reserpine; Dextrans; Kinetics; Recombinant Fusion Proteins; Amphetamine; Mice, Inbred BALB C; Presynaptic Terminals; Adrenergic Fibers; Norepinephrine Plasma Membrane Transport Proteins