Journal article icon

Journal article

Nuclear distribution and chromatin association of DNA polymerase α-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast

Abstract:

Background: Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. Results: Insertion of a TEV protease cleavage site into Cdc23 allows in vivo removal of the C-terminal 170 aa of the protein by TEV protease induction, resulting in an S phase arrest. This C-terminal fragment of Cdc23 is not retained in the nucle...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.1186/1471-2199-6-13

Authors


More by this author
Institution:
University of Oxford
Department:
Medical Sciences Division - Clinical Medicine,Nuffield Department of
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Zoology
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Zoology
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Zoology
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Zoology
Role:
Author
Cancer Research UK More from this funder
Publisher:
Biomed Central Ltd. Publisher's website
Journal:
BMC Molecular Biology Journal website
Volume:
6
Pages:
Article number 13
Publication date:
2005-06-05
DOI:
ISSN:
1471-2199
URN:
uuid:dcb273b3-9753-4f0b-b4be-7acd4809d477
Local pid:
ora:1687
Language:
English
Subjects:

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP