Journal article icon

Journal article

Monitoring conformational changes in the NDM-1 metallo-β-lactamase by 19F NMR spectroscopy.

Abstract:

The New Delhi metallo-β-lactamase (NDM-1) is involved in the emerging antibiotic resistance problem. Development of metallo-β-lactamases (MBLs) inhibitors has proven challenging, due to their conformational flexibility. Here we report site-selective labeling of NDM-1 with 1,1,1-trifluoro-3-bromo acetone (BFA), and its use to study binding events and conformational changes upon ligand-metal binding using (19) F NMR spectroscopy. The results demonstrate different modes of binding of known NDM-1...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1002/anie.201310866

Authors


Rydzik, AM More by this author
van Berkel, SS More by this author
Pfeffer, I More by this author
Expand authors...
Publisher:
Wiley-VCH Verlag
Journal:
Angewandte Chemie (International ed. in English)
Volume:
53
Issue:
12
Pages:
3129-3133
Publication date:
2014-03-05
DOI:
EISSN:
1521-3773
ISSN:
1433-7851
URN:
uuid:dc9a3d57-4967-4f40-a35c-0e3e79bae70a
Source identifiers:
457802
Local pid:
pubs:457802

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP