Effector/memory T cells of the weanling mouse exhibit Type 2 cytokine polarization in vitro and in vivo in the advanced stages of acute energy deficit.

Steevels, T; Hillyer, L; Monk, J; Fisher, M; Woodward, B

Journal article

Effector/memory T cells of the weanling mouse exhibit Type 2 cytokine polarization in vitro and in vivo in the advanced stages of acute energy deficit.

Abstract:: Our objective was to determine whether the polarizing cytokine profile of the effector/memory T-cell compartment reflects the profound decline of cell-mediated inflammatory competence that characterizes acute prepubescent malnutrition. Weanling C57BL/6J mice were permitted free access to a complete purified diet, free access to an isocaloric low-protein diet or restricted intake of the complete diet for 14 days. First, interleukin (IL)-4 and interferon (IFN)-gamma concentrations generated in vitro by splenic and nodal effector/memory T cells were assessed following exposure to plate-bound anti-CD3. Second, net systemic production of IFN-gamma and IL-4 by the effector/memory T-cell compartment was assessed by the in vivo cytokine capture assay following anti-CD3 stimulation. In vitro stimulation generated less IFN-gamma (P=.002) but more IL-4 (P=.05) by T cells from the restricted-intake group relative to the age-matched control group. Similarly, in vivo stimulation generated low serum levels of antibody-captured IFN-gamma in the restricted-intake group vis-à-vis the age-matched control group (P=.01), while the IL-4 response was sustained (P=.39). By contrast, the 14-day low-protein model exhibited no change in T-cell cytokine signature either in vitro or in vivo. However, following extended consumption of the low-protein diet (26 days), carcass energy losses exceeded those of the 14-day protocol and serum levels of in vivo antibody-captured IFN-gamma were low after anti-CD3 challenge relative to the age-matched control group (P=.02), while levels of captured IL-4 remained unaffected (P=.07). Acute weanling malnutrition elicits a Type 2 polarizing cytokine character on the part of the effector/memory T-cell compartment, but only in the most advanced stages of energy decrement.

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APA Style

Steevels, T., Hillyer, L., Monk, J., Fisher, M., & Woodward, B. (2010). Effector/memory T cells of the weanling mouse exhibit Type 2 cytokine polarization in vitro and in vivo in the advanced stages of acute energy deficit. Journal of Nutritional Biochemistry, 21(6), 504–511.

MLA Style

Steevels, T., et al. “Effector/Memory T Cells of the Weanling Mouse Exhibit Type 2 Cytokine Polarization in Vitro and in Vivo in the Advanced Stages of Acute Energy Deficit.” Journal of Nutritional Biochemistry, vol. 21, no. 6, 2010, pp. 504–11.

Chicago Style

Steevels, T, L Hillyer, J Monk, M Fisher, and B Woodward. 2010. “Effector/Memory T Cells of the Weanling Mouse Exhibit Type 2 Cytokine Polarization in Vitro and in Vivo in the Advanced Stages of Acute Energy Deficit.” Journal of Nutritional Biochemistry 21 (6): 504–11.
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