Journal article icon

Journal article

A recombinant vaccinia virus based ELISPOT assay detects high frequencies of Pol-specific CD8 T cells in HIV-1-positive individuals.

Abstract:

OBJECTIVES: HIV-1-specific CD8 T cells are considered to be critical in anti-HIV responses. It is important to quantify these cells and to determine their antigenic targets. Here quantification of interferon (IFN)-gamma secreting, virus-specific cells was achieved with an enzyme linked immuno spot (ELISPOT) assay. METHODS: Peripheral blood mononuclear cells (PBMC) were infected with recombinant vaccinia vectors expressing HIV-1 genes (gag, pol, env or nef) and added to wells precoated with an...

Expand abstract
Publication status:
Published

Actions


Access Document


Authors


Larsson, M More by this author
Ramratnam, B More by this author
Engelmayer, J More by this author
Expand authors...
Journal:
AIDS (London, England)
Volume:
13
Issue:
7
Pages:
767-777
Publication date:
1999-05-05
DOI:
EISSN:
1473-5571
ISSN:
0269-9370
URN:
uuid:dc37c992-ea9f-44d6-b212-417d381df522
Source identifiers:
25763
Local pid:
pubs:25763

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP