Optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples.

Journal article

In a study to optimize bacterial whole cell biosensors (bioreporters) for the detection of environmental contaminants, we constructed a toxicity sensing strain Acinetobacter baylyi ADP1_recA_lux. The ADP1_recA_lux is a chromosomally based bioreporter which makes the sensing system stable and negates the need for antibiotics to maintain the trait. The AOP1_recA_lux is activated to express bioluminescence when it is exposed to DNA damaging toxicants. Since the ADP1_recA_lux constantly expresses...

Expand abstract

Authors: Song, Y; Li, G; Thornton, S; Thompson, I; Banwart, SA

• Publication status: Published
• Journal: Environmental science and technology
• Volume: 43
• Issue: 20
• Pages: 7931-7938
• Publication date: 2009-10-01
• DOI: 10.1021/es901349r
• EISSN: 1520-5851
• ISSN: 0013-936X
• Language: English
• Keywords: Hazardous Substances; Genes, Reporter; Benzo(a)pyrene; DNA Damage; Acinetobacter; Toxicity Tests; Biosensing Techniques; Water Pollution, Chemical; Mitomycin