Detection of cartilage matrix degradation by autofluorescence lifetime.

Journal article

Cartilage is a vital organ to maintain joint function. Upon arthritis, proteolytic enzymes initiate degradation of cartilage extracellular matrix (ECM) resulting in eventual loss of joint function. However, there are only limited ways of non-invasively monitoring early chemical changes in cartilage matrix. Here we report that the autofluorescence decay profiles of cartilage tissue are significantly affected by proteolytic degradation of cartilage ECM and can be characterised by measurements o...

Expand abstract

Authors: Manning, H; Nickdel, M; Yamamoto, K; Lagarto, J; Kelly, D

• Publication status: Published
• Journal: Matrix biology : journal of the International Society for Matrix Biology
• Volume: 32
• Issue: 1
• Pages: 32-38
• Publication date: 2013-01-01
• DOI: 10.1016/j.matbio.2012.11.012
• EISSN: 1569-1802
• ISSN: 0945-053X
• Language: English
• Keywords: Animals; Cartilage; Biological Markers; Proteolysis; Optical Imaging; Extracellular Matrix Proteins; Swine; Trypsin; Fluorometry; Collagenases; Cattle; Extracellular Matrix